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The method of 2D cell culture is not a true reflection of the physiological cell environment where cells connect to other cells and, also to the extracellular matrix (ECM). Therefore some of the processes studied in 2D culture such as gene expression, apoptosis and importantly drug uptake and toxicity may not be directly transferable to in vivo experiments. HCS Pharma has developed a physiological and natural hydroscaffold, based on Hyaluronic Acid (HA) biofunctionalized with ECM components as collagens I, IV or VI, fibronectin and other molecules of interest. We will present the use of this scaffold on an automated platform, with the development of a 3D cell model for steatosis and phospholipidosis assessment as an example. Automated HepG2 cells seeding, medium renewal, spheroids treatment, fixation, staining and imaging will be discussed.
BIOMIMESYS® represents a new generation of mimetic hydroscaffold for 3D cell culture. Available in a ready-to-use format it enables the culture of cells under physiological conditions that are representative of the microenvironment found in whole tissues. The highly porous nature of the hydroscaffold allows the rapid uptake of nutrients, oxygen, etc. into the cells to create a reproducible study model for all downstream analytical techniques used with 3D cell culture.
Biomimesys® scaffolds technology is an innovative tool, closely mimeting the ECM matrix for relevant 3D cell culture allowing advanced drug discovery, ADME-Tox profiling and disease modelling. It is physiological, easy to use and compatible with all standard analysis methods (fluorescent plate reader, microscopy, Immunofluorescence, direct cell lysis for nucleic acids and protein extraction, histology).
View and download on Slideshare (low quality) : https://www.slideshare.net/hcspharma/biomimesys-hydroscaffold-for-3d-cell-culture-automation-of-the-processes-from-cell-seeding-to-imaging