Tumour cells will be grown as monolayer at 37°C in a humidified atmosphere (5% CO2, 95% air). DMEM (ref: BE12-604F, Lonza, Verviers, Belgium) supplemented with 10% fetal bovine serum (ref: 3302, Lonza) and 1% penicillin streptomycin (ref: DE-17-602F, Lonza, Verviers, Belgium). The cells are adherent to plastic flasks. For experimental use, tumour cells will be detached from the culture flask by a 5 minute treatment with trypsin-versene (ref: BE02-007E, Lonza) at 37°C, in Hanks’ medium without calcium or magnesium (ref: BE10-543F, Lonza) and neutralized by addition of complete culture medium. The cells will be counted in a hemocytometer and their viability will be assessed by 0.25% trypan blue exclusion assay.
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