Protocols

Immunofluorescent markers

BIOMIMESYS® allows cell culture in 3 dimensions and their observation by fluorescence microscopy without changing the labelling and observation protocols commonly used. It is possible to place the hydroscaffold in a test tube for fluorescence labelling. This allows washing and aspiration of the hydroscaffold safely (see diagram below) and requires Read more…

Protocols

Evaluation of BIOMIMESYS® hydroscaffold in mice bearing subcutaneous HCT 116 Human Colorectal tumour cells

Tumour cells will be grown as monolayer at 37°C in a humidified atmosphere (5% CO2, 95% air). DMEM (ref: BE12-604F, Lonza, Verviers, Belgium) supplemented with 10% fetal bovine serum (ref: 3302, Lonza) and 1% penicillin streptomycin (ref: DE-17-602F, Lonza, Verviers, Belgium). The cells are adherent to plastic flasks. For experimental Read more…

Protocols

Flow cytometry

Measurement of cell density and type to determine the cell cycle phase. Flow cytometry enables the physical separation of cells based on size, morphology and specific markers enabling multiple data to be obtained from one experiment. Download the complete PDF document here : FLOW CYTOMETRY_20180515

Protocols

Microscopy

A microscope is the piece of equipment most regularly used in the laboratory to verify and follow-up cell-culture experiments. In 3D, the adoption of focal plane merging or Z-stacking, becomes a necessity, this is because 3D brings a new dimension compared with 2D; the field depth. The use of microscopic Read more…

Protocols

Protein Extraction

Measurement of protein expression (Western blot) within a cell culture by protein extraction. The amount of protein produced by cells can be measured by antibody detection. Western blot can be used to detect the protein in a semi-quantifiable manner by interaction with a specific antibody. Analysis of protein expression in Read more…

Protocols

RNA or DNA extraction

Measurement of gene expression within a cell culture. The extraction of mRNA from the cells grown in BIOMIMESYS® enables analysis of gene expression to determine the physiological characteristics of the cells. Download the complete PDF document here : RNA OR DNA EXTRACTION_20180515