SEM observation of a BIOMIMESYS® Oncology: section magnification

SEM observation of a BIOMIMESYS® Oncology: section magnification

Extracellular matrix (ECM) is a complex viscoelastic system composed of various distinct components (GAG, Collagen…) that are often organized into a fibrillar microstructure.

The physicochemical properties (composition and architecture) of the ECM play a critical role in regulating important cellular functions such as spreading, migration, proliferation, and differentiation.

To better mimic the tumor microenvironment, HCS Pharma develops BIOMIMESYS® Oncology , a double interpenetrating network (IPN) hydroscaffold based on Hyaluronic acid (HA) and type I collagen crosslinked with Adipic acid dihydrazid (ADH).

HTC-116 Cells – Day 30 – DAPI (blue) MitoTracker (Yellow) Edu (Green) merged colors – 10 deep layers view

Spheroid growth, Viability, Cell proliferation

BIOMIMESYS® Oncology  hydroscaffold provides cell binding sites with CD44 and RHAMM allowing cell-matrix and cell-cell interactions.

Colorectal cancer cells grown in BIOMIMESYS® Oncology

Colorectal cancer cells grown in BIOMIMESYS® Oncology

Cancer cells growing in BIOMIMESYS® Oncology hydrscaffold proliferate and aggregate to form spheroids from the 5th day of culture. The size and formation of the spheroids varies as a function of:

  • Seeding density
  • Length of culture
  • Cell type

The cells can be cultured for at least 28 days.

HT-29 Cells - Day 7 - DAPI (blue) Mitotraker (Yellow)

HT-29 Cells – Day 7 – DAPI (blue) MitoTracker (Yellow)

HTC-116 Cells - Day 15 - DAPI (blue) MitoTracker (Yellow) Edu (Green)

HTC-116 Cells – Day 15 – DAPI (blue) MitoTracker (Yellow) Edu (Green)

HTC-116 Cells - Day 30 - DAPI (blue) MitoTracker (Yellow) Edu (Green) and merged colors

HTC-116 Cells – Day 30 – DAPI (blue) MitoTracker (Yellow) Edu (Green) and merged colors