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  • Immunofluorescent markers
    BIOMIMESYS® allows cell culture in 3 dimensions and their observation by fluorescence microscopy without changing the labelling and observation protocols commonly used. It is possible to place the hydroscaffold in a test tube for fluorescence labelling. This allows washing and aspiration of the hydroscaffold safely (see diagram below) and requires […]
  • Evaluation of BIOMIMESYS® hydroscaffold in mice bearing subcutaneous HCT 116 Human Colorectal tumour cells
    Tumour cells will be grown as monolayer at 37°C in a humidified atmosphere (5% CO2, 95% air). DMEM (ref: BE12-604F, Lonza, Verviers, Belgium) supplemented with 10% fetal bovine serum (ref: 3302, Lonza) and 1% penicillin streptomycin (ref: DE-17-602F, Lonza, Verviers, Belgium). The cells are adherent to plastic flasks. For experimental […]
  • Preparation of samples for histological labelling
    It is possible to make histological sections as well as perform Hematoxylin-Eosin-Saffron staining and/or immunohistochemical reactions on BIOMIMESYS®. This protocol can be used with any cell type grown in BIOMIMESYS®. It uses standard reagents for making a histological section but also more specific reagents such as OTTIX and OTTIX shaper […]
  • Flow cytometry
    Measurement of cell density and type to determine the cell cycle phase. Flow cytometry enables the physical separation of cells based on size, morphology and specific markers enabling multiple data to be obtained from one experiment. Download the complete PDF document here : FLOW CYTOMETRY_20180515
  • Microscopy
    A microscope is the piece of equipment most regularly used in the laboratory to verify and follow-up cell-culture experiments. In 3D, the adoption of focal plane merging or Z-stacking, becomes a necessity, this is because 3D brings a new dimension compared with 2D; the field depth. The use of microscopic […]
  • Protein Extraction
    Measurement of protein expression (Western blot) within a cell culture by protein extraction. The amount of protein produced by cells can be measured by antibody detection. Western blot can be used to detect the protein in a semi-quantifiable manner by interaction with a specific antibody. Analysis of protein expression in […]
  • RNA or DNA extraction
    Measurement of gene expression within a cell culture. The extraction of mRNA from the cells grown in BIOMIMESYS® enables analysis of gene expression to determine the physiological characteristics of the cells. Download the complete PDF document here : RNA OR DNA EXTRACTION_20180515
  • Preparation of samples for scanning electron microscopy (SEM)
    BIOMIMESYS® Hydroscaffold is compatible with scanning electron microscopy (SEM) analyses to investigate fine structural features of the hydroscaffold and cells grown inside. Two protocols can be carry out to have an optimal conservation of the hydroscaffold (part 1) or of the cells (part 2). Download the complete PDF document here […]
  • Protocol for metabolic activity determination
    Cell viability, as a result of various enzymatic activities in live cultured cells, can be measured by using reagents / substrates that convert into measurable products (particularly optical density, for e.g. WST1, Roche diagnostics), or MTT Protocol for metabolic activity determination
  • Retrieval of cells from BIOMIMESYS® hydroscaffolds
    BIOMIMESYS® technology is a Hyaluronic Acid-based hydroscaffold that allows physiological 3D cell culture. Cells can be easily retrieved with a quick digestion of the hydroscaffold. For a more efficient digestion, the enzymatic digestion is coupled to a mechanical action by continuous gentle shaking. This process does not impact the viability […]
  • First steps
    BIOMIMESYS® is a hydroscaffold of crosslinked Hyaluronic Acid (HA) chains for cell culture in 3D. The product is provided as a ready-to-use dehydrated hydroscaffold in each well of a low attachment 96-well plate. BIOMIMESYS® is highly porous and upon seeding, the cells spread homogeneously inside. Non-rehydrated BIOMIMESYS® should not be […]
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